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编制
果蝇
实时成像的胚胎使用悬滴协议
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Biology
The Preparation of
Drosophila
Embryos for Live-Imaging Using the Hanging Drop Protocol
Please note that all translations are automatically generated.
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编制
果蝇
实时成像的胚胎使用悬滴协议
DOI:
10.3791/1206-v
•
08:57 min
•
March 13, 2009
•
Bruce H. Reed
,
Stephanie C. McMillan
,
Roopali Chaudhary
1
Department of Biology
,
University of Waterloo
Chapters
00:00
Title
00:15
Introduction
01:52
Step 1: Collect Embryos
02:24
Step 2: Prepare a Dechorionation Slide
02:51
Step 3: Prepare Imaging Chamber
03:32
Step 4: Place Halocarbon Oil on a Coverslip
04:05
Step 5: Picking Embryos From Plates
04:38
Step 6: Place Embryos onto Tape
05:01
Step 7: Roll Embryos to Remove the Chorion
06:15
Step 8: Transfer Embryos to Halocarbon Oil
06:47
Step 9: Push Embryos to Bottom of Oil
07:14
Step 10: Invert Coverslip on Live Imaging Chamber
07:38
Step 11: Secure Coverslip with Tape
Summary
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一个简单,廉价,有效的方法是准备住成像分析果蝇胚胎。我们的协议提供湿度和气体交换,不压缩的果蝇胚胎。这种方法适用于基于GFP的使用立体显微镜或直立复式显微镜的果蝇胚胎的实时成像。
Tags
Drosophila Embryos
Live-imaging
Hanging Drop Protocol
GFP-based Timelapse
Genetic Regulation
Cell-cell Adhesion
Cell Death
Microscopy
Dehydration
Hypoxia
Halocarbon Oil
Gas-permeable Membrane
Coverslip
Compression
Biomechanical-based Analysis
Viability
Halocarbon Oil
Humid Chamber
Gas Exchange
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