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编制果蝇实时成像的胚胎使用悬滴协议
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
DOI:

08:57 min

March 13, 2009

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Chapters

  • 00:00Title
  • 00:15Introduction
  • 01:52Step 1: Collect Embryos
  • 02:24Step 2: Prepare a Dechorionation Slide
  • 02:51Step 3: Prepare Imaging Chamber
  • 03:32Step 4: Place Halocarbon Oil on a Coverslip
  • 04:05Step 5: Picking Embryos From Plates
  • 04:38Step 6: Place Embryos onto Tape
  • 05:01Step 7: Roll Embryos to Remove the Chorion
  • 06:15Step 8: Transfer Embryos to Halocarbon Oil
  • 06:47Step 9: Push Embryos to Bottom of Oil
  • 07:14Step 10: Invert Coverslip on Live Imaging Chamber
  • 07:38Step 11: Secure Coverslip with Tape

Summary

Automatic Translation

一个简单,廉价,​​有效的方法是准备住成像分析果蝇胚胎。我们的协议提供湿度和气体交换,不压缩的果蝇胚胎。这种方法适用于基于GFP的使用立体显微镜或直立复式显微镜的果蝇胚胎的实时成像。

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