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斑马鱼脑室注射液
JoVE Journal
Biology
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JoVE Journal
Biology
Zebrafish Brain Ventricle Injection
Please note that all translations are automatically generated.
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斑马鱼脑室注射液
DOI:
10.3791/1218-v
•
08:28 min
•
April 06, 2009
•
Jennifer H. Gutzman
,
Hazel Sive
2
1
Whitehead Institute for Biochemical Research
,
2
MIT – Massachusetts Institute of Technology
Chapters
00:00
Title
00:44
Introduction
01:33
Preparing the needles for microinjection
02:18
Preparing the embryos
03:41
Injecting the brain ventricle
04:34
Imaging
05:41
Image processing
06:37
Representative Results/Outcome
07:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
神经管形成后,神经上皮收缩和皱褶而胚胎脑脊液(eCSF)形成的胚胎脑室管填充。我们开发这个脑室注射技术,以更好地可视化的充满液体的空间,相反,在一个活的胚胎神经上皮形状。
Tags
Zebrafish
Brain Ventricle
Embryonic Brain Development
Cerebrospinal Fluid
Neuroepithelium
Ventricle Formation
Visualization
Fluorescent Dye
Brightfield Microscopy
Fluorescent Microscopy
Photoshop
Neuroepithelial Shape Changes
Brain Morphogenesis Mutants
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