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Journal
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Biology
/
使用哺乳动物骨骼肌的DNA转染
在体内
电穿孔
JoVE Journal
Biology
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JoVE Journal
Biology
DNA Transfection of Mammalian Skeletal Muscles using
In Vivo
Electroporation
Please note that all translations are automatically generated.
Click here for the English version.
使用哺乳动物骨骼肌的DNA转染
在体内
电穿孔
DOI:
10.3791/1520-v
•
15:56 min
•
October 19, 2009
•
Marino DiFranco
,
Marbella Quinonez
,
Joana Capote
,
Julio Vergara
1
Department of Physiology
,
David Geffen School of Medicine, University of California, Los Angeles
Chapters
00:00
Title
01:16
Introduction
01:49
Plasmid Preparation for Electroporation
02:46
Animal Preparation for Electroporation
06:12
Electroporation and Transfection
08:31
Assaying In-Vivo Transfection by Electroporation
08:36
Transfection Efficiency
09:47
Subcellular Protein Localization
12:31
Expression and Targeting of the a-sub-unit of the Dihydropyridine Receptor (DHPR-a1s)
13:56
Functional Evaluation of the Expression of EYFP-CIC1
15:30
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
我们描述了使用电和随后的可视化的表达蛋白,用荧光显微镜活体小鼠的脚部肌肉纤维的高效转染质粒DNA的详细程序。
Tags
DNA Transfection
Mammalian Skeletal Muscles
In Vivo Electroporation
Cell Biology
Transgenic Proteins
Fluorescently Tagged Constructs
Mutant Variants
In Vivo Transfection
Plasmids
Liver
Skeletal Muscle
Brain
Flexor Digitorum Brevis
Interosseus Muscles
Adult Mice
Experimental Parameters
Soluble Proteins
Structural Proteins
Membrane Proteins
EGFP
ECFP3
Calpain
FKBP12
β2a-DHPR
Minidystrophin
α-actinin
α1s-DHPR
RyR1
Cardiac Na/Ca2+ Exchanger
NaV1.4 Na Channel
SERCA1
Article
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