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Memeli İskelet Kaslar kullanarak DNA Transfeksiyon In Vivo Elektroporasyon
JoVE Journal
Biology
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JoVE Journal Biology
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
DOI:

15:56 min

October 19, 2009

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Chapters

  • 00:00Title
  • 01:16Introduction
  • 01:49Plasmid Preparation for Electroporation
  • 02:46Animal Preparation for Electroporation
  • 06:12Electroporation and Transfection
  • 08:31Assaying In-Vivo Transfection by Electroporation
  • 08:36Transfection Efficiency
  • 09:47Subcellular Protein Localization
  • 12:31Expression and Targeting of the a-sub-unit of the Dihydropyridine Receptor (DHPR-a1s)
  • 13:56Functional Evaluation of the Expression of EYFP-CIC1
  • 15:30Conclusion

Summary

Automatic Translation

Biz elektroporasyon ve floresan mikroskobu kullanarak protein ekspresyonu sonraki görselleştirme kullanılarak canlı farelerin ayak kaslarının lifleri plazmid DNA verimli transfeksiyon ayrıntılı prosedürler açıklanmaktadır.

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