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小鼠胚胎生殖细胞的分离和推导
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Isolation and Derivation of Mouse Embryonic Germinal Cells
DOI:

14:01 min

October 22, 2009

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Chapters

  • 00:00Title
  • 01:40Introduction
  • 02:14Pregnant Mouse Laparotomy
  • 05:04Gonadal Ridge Dissection
  • 08:35Gonadal Ridge Digestion
  • 11:03Primordial Germinal Cell Culture
  • 12:51Embryonic Germinal Cells
  • 13:26Conclusion

Summary

Automatic Translation

胚胎生殖细胞分化成原始生殖细胞在早期发展阶段的能力,是一个完美的模型,以解决我们的假设,对癌症和不育症。该协议显示了如何从发展中国家性腺的原始生殖细胞中分离出来后10.5-11.5天coitum小鼠胚胎。

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