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为贴壁细胞培养的氧气插入的制备及操作
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
Please note that all translations are automatically generated.
Click here for the English version.
为贴壁细胞培养的氧气插入的制备及操作
DOI:
10.3791/1695-v
•
11:56 min
•
January 06, 2010
•
Shawn Oppegard
,
Elly Sinkala
,
David Eddington
1
Bioengineering
,
University of Illinois
Chapters
00:00
Title
00:49
Introduction
01:21
Material Fabrication and Assembly of the Oxygenating Insert
03:57
Validation of Oxygenating Insert- Calibration, Heterogeneity and Equilibration.
07:45
Assembly of Oxygenating Insert for Wound Healing Cell Culture
09:56
Validating and Using an Oxygenating Insert in Monolayer Wound Healing
10:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
制造和验证一个附加的平台,在空间和时间在6孔板充氧提供增强的控制。该设备是适应了文化系统,可用于调查的氧气对伤口愈合的影响。
Tags
Fabrication
Operation
Oxygen Insert
Adherent Cellular Cultures
Oxygen Modulation
Biomedical Research
Hypoxic Chamber
Temporal Control
Spatial Control
Physiological Phenomena
Specialized Knowledge
Equipment
Microfabricated Insert
Multiwell Plates
Polydimethylsiloxane Insert
Passive Microfluidic Gas Network
Gas-permeable Membrane
Oxygen Delivery
Gas Cylinders
Fabrication Techniques
SU-8 Photolithography
Replica Molding
PDMS Spinning
Silicon Wafer
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