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脂質中間相を用いた構造決定のための膜タンパク質の結晶化
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Biology
Author Produced
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JoVE Journal
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Please note that all translations are automatically generated.
Click here for the English version.
脂質中間相を用いた構造決定のための膜タンパク質の結晶化
DOI:
10.3791/1712-v
•
22:00 min
•
November 21, 2010
•
Martin Caffrey
,
Christopher Porter
1
Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine
,
Trinity College Dublin
Chapters
00:00
Introduction
02:48
Materials
03:48
Preparing the Crystallization Plate
05:12
Preparing the Lipid and Protein
13:53
Loading the Dispensing Syringe
17:57
Dispensing
21:10
Crystals
Summary
Automatic Translation
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Automatic Translation
ここで脂質中間相で手動での膜タンパク質の結晶化の試験を設定するにはカフリーの膜構造機能生物学研究グループに実装されたプロシージャが記述されています。
Tags
Crystallizing Membrane Proteins
Lipidic Mesophases
Lipidic Cubic Phase
In Meso Method
X-ray Crystallographic Structures
Prokaryotic Proteins
Eukaryotic Proteins
Monomeric Proteins
Homo-multimeric Proteins
Hetero-multimeric Proteins
Chromophore-containing Proteins
Chromophore-free Proteins
Alpha-helical Proteins
Beta-barrel Proteins
Beta2-adrenergic Receptor
Adenosine A2a Receptor
Reconstituting Membrane Protein
Monoolein-based Mesophase
Manual Crystallization Setup
Crystal Harvesting
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