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Optische Scatter Microscopie op basis van twee-dimensionale Gabor filters
JoVE Journal
Biology
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JoVE Journal Biology
Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
DOI:

14:58 min

June 02, 2010

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Chapters

  • 00:00Title
  • 00:50Introduction
  • 01:26Protocol
  • 02:18Preparing the Optical Setup
  • 05:26Loading the Filterbank and Using the Setup to Acquire Filtered-Background Images
  • 06:57Plating the Cells
  • 09:44Performing Optical Scatter Microscopy
  • 11:53Representative Images
  • 14:28Conclusion

Summary

Automatic Translation

We zien een donker veld microscopie op basis van Gabor-achtige filter om subcellulaire dynamiek maatregel binnen enkele levende cellen. De techniek is gevoelig voor veranderingen in de structuur van de organellen, zoals mitochondriën fragmentatie.

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