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真核Polyribosome剖面分析
JoVE Journal
Biology
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JoVE Journal Biology
Eukaryotic Polyribosome Profile Analysis
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真核Polyribosome剖面分析

DOI:
10.3791/1948-v

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09:16 min

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June 15, 2010

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, , , , , , ,
1Department of Molecular Genetics, Microbiology, and Immunology,University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School

Chapters

  • 00:45Introduction
  • 01:15Preparation of Sucrose Gradients
  • 02:53Preparation of Extracts from Yeast Cells
  • 05:06Centrifugation of Gradients and Collection of Data and Fractions
  • 08:35Representative Trace of a Ribosome Extract
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了协议的真核细胞的核糖体翻译的提取。一旦提取，核糖体是由蔗糖梯度分馏分离到monosomes和多聚核糖体，允许不同的核糖体的人群进行分析。因此，这种方法是研究翻译调控的黄金标准。

Tags

EukaryoticPolyribosome Profile AnalysisProtein SynthesisTranslation RegulationMRNA/ribosome ComplexesSucrose Density GradientPolyribosomesMonosomesTranslation InitiationElongation/terminationMRNA CohortExperimental ConditionsRibosome AssemblySmall Ribosomal SubunitLarge Ribosomal Subunit

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