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JoVE Journal
Biology

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真核Polyribosome剖面分析
 
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真核Polyribosome剖面分析

Article DOI: 10.3791/1948
June 15th, 2010

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Summary June 15th, 2010

Please note that all translations are automatically generated.

Click here for the English version.

本文介绍了协议的真核细胞的核糖体翻译的提取。一旦提取,核糖体是由蔗糖梯度分馏分离到monosomes和多聚核糖体,允许不同的核糖体的人群进行分析。因此,这种方法是研究翻译调控的黄金标准。

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