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Journal
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Biology
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Large-Scale Screens of Metagenomic Libraries
JoVE Journal
Biology
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JoVE Journal
Biology
Large-Scale Screens of Metagenomic Libraries
Large-Scale Screens of Metagenomic Libraries
DOI:
10.3791/201-v
•
16:05 min
•
May 28, 2007
•
Vinh D. Pham
,
Tsultrim Palden
,
Edward F. DeLong
1
Division of Biological Engineering, Department of Civil and Environmental Engineering
,
MIT – Massachusetts Institute of Technology
Chapters
00:02
Membrane filters
00:48
Large-scale inoculation of bacterial colonies on membrane
03:00
Fixing colony DNA on membrane
05:55
Hybridization
08:10
Washing membranes after hybridization
10:49
Detection of the signal
Tags
Metagenomic Libraries
Large-scale Screens
Contiguous Genomic Sequences
Microorganisms
Prior Cultivation
Streamlined Procedure
Screening Metagenomic Libraries
High-throughput Investigations
Genetic Properties
Metabolic Properties
Uncultured Microbial Assemblages
Key Protocols
Video Format
Robotic Instrumentation
Manual Interventions
Library Clones
High-density Macroarray Membranes
Automation
Accuracy
Speed
Miniaturization Of Scale
Spatial Arrangements
Probe Labeling
Membrane Hybridization
Signal Detection
Visual Demonstration
Written Descriptions
Article
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