Journal
/
Biology
/
Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

DOI:
10.3791/2012-v

•

05:45 min

•

April 26, 2010

•

, , ,
1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Department of Physics, Lewis-Sigler Institute for Integrative Genomics,Princeton University

Chapters

  • 00:00Title
  • 00:41Introduction
  • 01:12Attaching Bacteria to a Coverlip and Preparing the Beads
  • 03:19Measuring the Bending Stiffness of the Bacterial Cells
  • 05:11Measuring the Bending Stiffness of Bacterial Cells Representative Results
  • 05:21Conclusion
  • 05:44Credits

Summary

Automatic Translation

Automatic Translation

We present a protocol for bending filamentous bacterial cells attached to a cover-slip surface with an optical trap to measure the cellular bending stiffness.

Tags

MeasuringBending StiffnessBacterial CellsOptical TrapProtocolFilamentous Rod-shaped BacteriaForcesMicroscopic Three-dimensional SpringHigh-intensity Laser BeamChemically-treated CoverslipLive BacteriaGrowing EndsFilamentous GrowthDrug CephalexinCell AttachmentBending ForcesPolylysine-coated BeadForce MeasurementDisplacement Measurement

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2012?article_type=v

Proper Care and Cleaning of the Microscope
check_url/2012?article_type=v

Major Components of the Light Microscope
check_url/2012?article_type=v

Electron Cryotomography of Bacterial Cells
check_url/2012?article_type=v

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
check_url/2012?article_type=v

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
check_url/2012?article_type=v

Measuring the Kinetics of mRNA Transcription in Single Living Cells
check_url/2012?article_type=v

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay
check_url/2012?article_type=v

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
check_url/2012?article_type=v

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
check_url/2012?article_type=v

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Read Article