A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 01:15Introduction
- 01:53Overview of the Integrated Microscope System
- 03:26Preparation for AFM
- 06:37Mechanical Stimulation of the Live Cell
- 07:43Representative Results
- 08:48Conclusion
Detta dokument syftar till att instruera läsaren i driften av ett integrerat atomic force-optisk avbildning mikroskop för mekanisk stimulering av levande celler i kultur. En steg-för-steg-protokollet presenteras. En representant datauppsättning som visar levande cell svar på mekanisk stimulering presenteras.
Tags
Live Cell ResponseMechanical StimulationIntegrated Optical MicroscopyAtomic Force MicroscopySub-cellular LevelSpatial ResolutionTemporal ResolutionTotal Internal Reflection Fluorescence MicroscopyFast-spinning Disk Confocal MicroscopyMolecular Dynamic StudiesAdherent Live CellsOptical ImagingReal-timeActin FilamentsFocal AdhesionsCellular StructureCell RestructuringDynamicsDetailed Protocol
