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差分成像生物结构与双共振相干反斯托克斯拉曼散射(CARS)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
Please note that all translations are automatically generated.
Click here for the English version.
差分成像生物结构与双共振相干反斯托克斯拉曼散射(CARS)
DOI:
10.3791/2085-v
•
12:56 min
•
October 17, 2010
•
Tyler J. Weeks
,
Thomas R. Huser
2
1
Center for Biophotonics
,
University of California, Davis
,
2
Department of Internal Medicine
,
University of California, Davis
Chapters
00:00
Title
00:46
Introduction
01:21
Overview of the DR-CARS Microscopy System
05:22
The Generation of CARS and Doubly-resonant CARS Signals
07:02
Sample Preparation
11:16
Imaging Results
12:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
结合三个单一波长的短脉冲激光器是用来产生相干反斯托克斯拉曼散射(CARS)和双谐振车(DR车)。这些信号之间的区别,否则很难察觉连贯的拉曼信号增强的灵敏度,使弱拉曼散射成像。
Tags
Differential Imaging
Biological Structures
Doubly-resonant
Coherent Anti-stokes Raman Scattering (CARS)
Coherent Raman Imaging
Label-free Optical Imaging
Molecular Specificity
Sensitivity
Fluorescence
Weak Concentrations
Raman-active Molecules
Signal Amplification
Pulsed Lasers
Coherent Raman Scattering Signals
Coherent Anti-stokes Raman Scattering (CARS)
3-color CARS
Four-wave Mixing (FWM)
Doubly-resonant CARS (DR-CARS)
Constructive Interference
Tuning Lasers
CH Stretch Vibration
CD Stretching Vibration
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