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Chapters
- 00:00Title
- 00:41Introduction
- 01:06Tissue Preparation and Mounting
- 02:07Optimization of the LAESI Ion Source
- 05:54Molecular Imaging and Data Analysis
- 07:49Mass Spectrometry of Biological Tissues Representative Results
- 08:44Conclusion
Laser ablation electrospray ionization (LAESI) is an atmospheric-pressure ion source for mass spectrometry. In the imaging mode, a mid-infrared laser probes the distributions of molecules across a tissue section or a biofilm. This technique presents a new approach for diverse bioanalytical studies carried out under native experimental conditions.
Tags
Atmospheric-pressure Molecular ImagingBiofilmsLAESI Mass SpectrometryAmbient Ionization MethodsLaser Ablation Electrospray IonizationWater-containing SpecimensMid-infrared Laser BeamAblation Fluence ThresholdParticulate MatterAblation PlumeHighly Charged DropletsGas-phase IonsMass SpectrometerAtmospheric-pressure Ion Source InterfaceMolecular ImagingMicroprobe Analysis ModeDepth ProfilingLateral ImagingThree-dimensional (3D) Molecular ImagingLateral ResolutionDepth Resolution
