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Journal
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Immunology and Infection
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弓形虫
囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Toxoplasma gondii
Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
Please note that all translations are automatically generated.
Click here for the English version.
弓形虫
囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件
DOI:
10.3791/2091-v
•
09:56 min
•
August 12, 2010
•
Crystal Tobin
,
Angela Pollard
,
Laura Knoll
1
Department of Medical Microbiology and Immunology
,
University of Wisconsin
Chapters
00:04
Title
00:44
Introduction
01:10
Preparation of HFF-coated Coverslips
02:37
Infecting Cells
03:57
Detection of Bradyzoites
05:48
Inducing Cyst Wall Formation
09:08
Representative Results
09:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
弓形虫囊肿的形式,在应对环境压力,可在组织培养模式模仿的转换。本视频演示了技术审查囊肿壁的形成,激活骨髓来源的巨噬细胞或改变成纤维细胞生长介质的pH值。
Tags
Toxoplasma Gondii
Cyst Wall Formation
Bone Marrow-derived Macrophages
Bradyzoite Conditions
Obligate Intracellular Parasite
Tachyzoite
Encysted Form
Signaling Process
Chronic Stage Of Infection
CO2 Starvation
High PH Medium
Interferon Gamma (IFNγ)
Dolichos Biflorus Agglutinin (DBA)
Fluorescently Labeled DBA
Human Foreskin Fibroblasts (HFFs)
Murine Bone Marrow-derived Macrophages (BMMs)
Lipopolysaccharide (LPS)
High PH Growth Medium
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