JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
小学组织两个胸苷类似物的免疫荧光检测(CldU IDU)
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
Please note that all translations are automatically generated.
Click here for the English version.
小学组织两个胸苷类似物的免疫荧光检测(CldU IDU)
DOI:
10.3791/2166-v
•
10:55 min
•
December 07, 2010
•
Alex H. Tuttle*
1
,
Matthew M. Rankin*
1
,
Monica Teta
,
Daniel J. Sartori
,
Geneva M. Stein
,
Gina J. Kim
,
Cristina Virgilio
,
Anne Granger
,
Di Zhou
,
Simon H. Long
,
Alisa B. Schiffman
,
Jake A. Kushner
1
Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics
,
University of Pennsylvania-School of Medicine
Chapters
00:01
Title
02:53
Primary and Secondary Antibodies
09:35
Imaging and Analysis
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们派生的策略,以检测到成年小鼠组织中的胸苷类似物的顺序法团(CldU IDU),以量化的细胞分裂的两个连续两轮。这一战略是有用的检测长寿命的组织细胞的营业额,致癌基因改造,或过境扩增细胞。
Tags
Immunofluorescent Detection
Thymidine Analogues
CldU
IdU
Cell Division Measurement
Experimental Biology
Microscopy
Vital Dyes
CFSE
Ki67
PCNA
Bromo-deoxyuridine (BrdU)
Chloro-deoxyuridine (CldU)
Iodo-deoxyuridine (IdU)
Ethinyl-deoxyuridine (EdU)
Radio-detection
Immuno-detection
Chemical Detection
Adult Mice
Immunostaining Protocols
Cell Turnover
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
原代培养及质粒电穿孔尔蒂小鼠器官。
大鼠肝细胞的分离和小学文化
从小鼠和人类结肠组织初级肌成纤维细胞的分离
瑞士改进轧制技术肠组织准备免疫组化和免疫分析
肌肉干细胞中自噬的
原位
免疫荧光染色
蝙蝠组织收集 -细胞分析和原细胞培养
使用两步PCR和下一代16S rRNA基因测序进行微生物群分析
海参肠细胞原培养的凋亡诱导与检测
通过免疫荧光简单检测原发性西莉亚
使用基于多反应监测的人类脑组织蛋白质的多反应监测工作流程定量蛋白质组学工作流程
Read Article