A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00The Method of Rodent Whole Embryo Culture Using the Rotator-type Culture System
- 00:07Introduction
- 02:06Setting up Whole Embryo Culture System
- 02:52Preparation of Culture Medium
- 03:53Removal of Embryos from the Uterus
- 04:52Dissection of Rat Embryos
- 07:27Results and Discussion
Whole Embryo Kultur Technik ermöglicht es uns, Kultur Maus und Ratte Embryonen<em> Ex vivo</em> Zustand während begrenzter Zeiträume entsprechend midgestation Stufen. In diesem Video-Protokoll, demonstrieren wir unsere Standard-Verfahren der Ratte ganze Embryo Kultur nach E12.5 mit dem Rotor-Typ Flasche Kultur-System.
Tags
Rodent Whole Embryo CultureWEC TechniqueRotator-type Bottle Culture SystemDevelopmental BiologyMouse EmbryosRat EmbryosEx Vivo ConditionEmbryonic DayPlacenta FunctionManipulation Under MicroscopeDynamic Developmental ProcessesPostimplanted Mammalian EmbryosLong-term CultureRat WEC After E12.5Refined ModelApplications In Mammalian Developmental Biology
