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Chapters
- 00:00The Method of Rodent Whole Embryo Culture Using the Rotator-type Culture System
- 00:07Introduction
- 02:06Setting up Whole Embryo Culture System
- 02:52Preparation of Culture Medium
- 03:53Removal of Embryos from the Uterus
- 04:52Dissection of Rat Embryos
- 07:27Results and Discussion
Whole technique de culture d'embryons de souris nous permet de culture et d'embryons de rat<em> Ex vivo</emCondition> durant des périodes limitées correspondant aux stades midgestation. Dans ce protocole vidéo, nous démontrons nos procédures standard de la culture d'embryon de rat entière après E12.5 en utilisant le système des rotateurs de type flacon de culture.
Tags
Rodent Whole Embryo CultureWEC TechniqueRotator-type Bottle Culture SystemDevelopmental BiologyMouse EmbryosRat EmbryosEx Vivo ConditionEmbryonic DayPlacenta FunctionManipulation Under MicroscopeDynamic Developmental ProcessesPostimplanted Mammalian EmbryosLong-term CultureRat WEC After E12.5Refined ModelApplications In Mammalian Developmental Biology
