Journal
/
/
Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
DOI:

07:59 min

December 05, 2020

, , , , , , , , , , , , , ,

Chapters

  • 00:05Introduction
  • 00:55Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
  • 02:23CSC Passage
  • 03:24HUEhT-1 Endothelial Cell Culture
  • 04:14NICO-1 Coculture Plate Preparation
  • 05:16NICO-1 System HUEhT-1 and CSC Seeding
  • 06:14Results: Representative CSC-Induced Neovascularization and Vascular Formation
  • 07:19Conclusion

Summary

Automatic Translation

Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro.

Related Videos

Read Article