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从噬菌体抗体库的肽调节间质细胞的基因表达和使可能在Unicortical骨缺损的成骨
JoVE Journal
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JoVE Journal
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Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
Please note that all translations are automatically generated.
Click here for the English version.
从噬菌体抗体库的肽调节间质细胞的基因表达和使可能在Unicortical骨缺损的成骨
DOI:
10.3791/2362-v
•
07:53 min
•
December 10, 2010
•
Gary Balian
,
Gina Beck
,
Vedavathi Madhu
,
Robert Sikes
,
Quanjun Cui
,
Haixiang Liang
,
Joshua Bush
1
Orthopaedics Research
,
University of Virginia
,
2
Biological Sciences
,
University of Delaware
,
3
Orthopaedic Surgery
,
University of Virginia
Chapters
00:05
Title
01:44
Biopanning
03:39
Peptide Staining of Mesenchymal Cultured Cells
04:42
Preparation of Gel Foam Composite and In Vivo Implantation
05:25
Histology
05:40
Effects of Phage Display Peptides on Osteogenesis In Vitro and In Vivo
06:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
噬菌体展示库,用于识别目标的骨肽序列。其目的是调查这些肽的间质细胞分化的影响,以确定其对骨再生的效果。
Tags
Peptides
Phage Display Library
Gene Expression
Mesenchymal Cells
Osteogenesis
Unicortical Bone Defects
Bone Formation
Collagen Matrix
Alkaline Phosphatase Activity
Nodule Formation
Runx2
Osterix
Bone Sialoprotein
Osteocalcin
Collagen Sponge
Repair Of Bone Defects
In Vitro Differentiation
In Vivo Delivery
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