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Chapters
- 00:02Title
- 00:05Introduction
- 01:30Using the Amaxa Nucleofector
- 10:35Viewing Transfection Efficiency
- 11:03Conclusion
Introducing a gene of interest into a cell is a powerful method for elucidating its function in vivo. This protocol describes an efficient method of transfecting a culture of human neural stem/precursor cells (hNSPCs) using the Nucleofector electroporation apparatus made by Amaxa.
Tags
TransfectingHuman Neural Stem CellsAmaxa NucleofectorTransfectionCationic Lipid Transfection ReagentsCell ViabilityTransfection EfficiencyGene GunMicroinjectionViral ConstructsExogenous GenePrimary CellsViral VectorsSafety ConcernsProtocolElectrical Current ParametersBuffer SolutionsStable TransfectionElectroporation
