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人类神经干细胞转染与Amaxa Nucleofector
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JoVE Journal Biology
Transfecting Human Neural Stem Cells with the Amaxa Nucleofector
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人类神经干细胞转染与Amaxa Nucleofector

DOI:
10.3791/240-v

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11:24 min

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July 19, 2007

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,
1Department of Pathology,University of California, Irvine (UCI)

Chapters

  • 00:02Title
  • 00:05Introduction
  • 01:30Using the Amaxa Nucleofector
  • 10:35Viewing Transfection Efficiency
  • 11:03Conclusion

Summary

Automatic Translation

Automatic Translation

感兴趣的基因引入细胞，为阐明其在体内的功能强大的方法。这个协议描述一个有效的方法转染人类神经干/前体细胞，使用由Amaxa Nucleofector电设备（hNSPCs）文化。

Tags

TransfectingHuman Neural Stem CellsAmaxa NucleofectorTransfectionCationic Lipid Transfection ReagentsCell ViabilityTransfection EfficiencyGene GunMicroinjectionViral ConstructsExogenous GenePrimary CellsViral VectorsSafety ConcernsProtocolElectrical Current ParametersBuffer SolutionsStable TransfectionElectroporation

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