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Neuroscience
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罗丹明-肌动蛋白标记F -肌动蛋白在通透的神经生长锥刺完
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
Please note that all translations are automatically generated.
Click here for the English version.
罗丹明-肌动蛋白标记F -肌动蛋白在通透的神经生长锥刺完
DOI:
10.3791/2409-v
•
09:14 min
•
March 17, 2011
•
Bonnie M. Marsick
,
Paul C. Letourneau
1
Department of Neuroscience
,
University of Minnesota
Chapters
00:05
Title
01:49
Preparation of Coverslips or “Video” Dishes
03:40
Preparation of Neuronal Cultures and Media
04:53
Permeabilization of Neuronal Cultures and Incorporation of Rhodamine-actin onto F-actin Barbed Ends
06:13
Variations to this Procedure
07:35
Representative Actin Labeling Results
08:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
描述的方法来可视化和量化,F -肌动蛋白在神经生长锥刺完。盖玻片上培养神经细胞后,细胞透用含皂甙的解决方案。然后,潜伏期短皂苷含有若丹明-肌动蛋白的缓冲区纳入到免费的肌动蛋白刺完肌动蛋白的荧光。
Tags
F-actin
Barbed Ends
Rhodamine-actin
Permeabilized
Neuronal Growth Cones
Axons
Molecular Guidance Cues
Growth Cone Cytoskeleton
Actin Filament Meshwork
Actin Polymerization
Dynamic Remodeling
Guidance Cues
Actin Monomer
Polymerization Nuclei
Chick Retinal
Dorsal Root Ganglion (DRG)
Actin Depolymerizing Factor (ADF/cofilin)
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