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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
JoVE Journal
Biology
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JoVE Journal Biology
Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
DOI:

09:24 min

July 29, 2007

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Chapters

  • 00:07Introduction
  • 00:46Growing Up Bacteria
  • 01:24Resuspending and Lysing Bacteria with Buffers P1 and P2
  • 03:56Precipitating Bacterial Protein with Buffer N3
  • 04:38Assembling the Qiavac Manifold during Centrifugation
  • 05:15Binding Plasmid DNA to Qiastrip and Washing with Buffers PB and PE
  • 07:19Eluting DNA in Collection Tubes
  • 08:48Conclusion

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