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从整个成年果蝇大脑细胞记录
JoVE Journal
Biology
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JoVE Journal Biology
Whole Cell Recordings from Brain of Adult Drosophila
DOI:

09:27 min

July 29, 2007

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Chapters

  • 00:04Title
  • 00:22Introduction
  • 00:53Preparation of Dissection Solution
  • 02:15Adult Drosophila Selection
  • 02:32Sex Differentiation
  • 03:04Decapitation and Removal of Brain
  • 04:19Removal of Trachea and Enzyme Treatment
  • 05:27Stabilizing Brain in Recording Chamber
  • 07:58Conclusion

Summary

Automatic Translation

这个视频演示隔离准备从单个神经元,使用标准的全细胞技术记录整个大脑在成年果蝇的过程。它包括绿色荧光标记的细胞和神经元在录制过程中查看的图像。

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