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从整个成年果蝇大脑细胞记录
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Biology
Whole Cell Recordings from Brain of Adult Drosophila
Please note that all translations are automatically generated.
Click here for the English version.
从整个成年果蝇大脑细胞记录
DOI:
10.3791/248-v
•
09:27 min
•
July 29, 2007
•
Huaiyu Gu
,
Diane K. O’Dowd
1
Department of Development and Cell Biology, Department of Anatomy and Neurobiology
,
University of California, Irvine (UCI)
Chapters
00:04
Title
00:22
Introduction
00:53
Preparation of Dissection Solution
02:15
Adult Drosophila Selection
02:32
Sex Differentiation
03:04
Decapitation and Removal of Brain
04:19
Removal of Trachea and Enzyme Treatment
05:27
Stabilizing Brain in Recording Chamber
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频演示隔离准备从单个神经元,使用标准的全细胞技术记录整个大脑在成年果蝇的过程。它包括绿色荧光标记的细胞和神经元在录制过程中查看的图像。
Tags
Whole Cell Recordings
Brain
Adult Drosophila
Procedure
Single Neurons
Dissecting Solution
Capture
Optic Lobes
Trachea
Isolated Brain
Neurons
Handling
Damage Prevention
Holder
Recording Chamber
Electrophysiology Setup
GAL4 Line
GFP Expression
Projection Neurons (PNs)
Live Brain
Nomarski Image
Targeted Neuron
Spontaneous Activity
Action Potentials
Synaptic Input
In Situ Preparation
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