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小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面
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Medicine
Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
Please note that all translations are automatically generated.
Click here for the English version.
小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面
DOI:
10.3791/2513-v
•
10:47 min
•
February 21, 2011
•
Hilaire C. Lam
2
,
Augustine M.K. Choi
,
Stefan W. Ryter
1
Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital
,
Harvard Medical School
,
2
Cellular and Molecular Pathology, School of Medicine
,
University of Pittsburgh
Chapters
00:00
Title
01:15
Dissection of Mouse Tracheas
03:40
Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
08:25
Application of Cigarette Smoke to Cultured Epithelial Cells
09:44
Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
10:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
从小鼠的呼吸道和肺上皮细胞可以分离,气液界面的差异化的呼吸道上皮模式培养。协议是分离,培养,这些细胞暴露卷烟主流烟雾,为了研究这种环境毒素的分子反应。
Tags
Mouse Respiratory Epithelial Cells
Isolation
Experimental Cigarette Smoke
Air-liquid Interface (ALI)
Differentiated Respiratory Epithelium
Mainstream Cigarette Smoke (CS) Exposure
Airway Epithelial Cells
Trachea
Fully Differentiated Epithelial Cells
Calibrated Mainstream CS Delivery
ALI Culture System
Respiratory Epithelia
Physiological Setting
Molecular Responses
Lung Cellular Responses
Environmental Air Pollution
Human Health
Chronic Obstructive Pulmonary Disease (COPD)
Tobacco-related Diseases
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