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Chapters
- 00:00Title
- 01:15Dissection of Mouse Tracheas
- 03:40Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
- 08:25Application of Cigarette Smoke to Cultured Epithelial Cells
- 09:44Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
- 10:28Conclusion
Células epiteliais pulmonares podem ser isolados do trato respiratório de camundongos e cultivadas em ar-líquido interface como um modelo de epitélio respiratório diferenciado. Um protocolo é descrito para o isolamento, cultura e expondo essas células à fumaça de cigarro mainstream, a fim de estudar as respostas moleculares para esta toxina ambiental.
Tags
Mouse Respiratory Epithelial CellsIsolationExperimental Cigarette SmokeAir-liquid Interface (ALI)Differentiated Respiratory EpitheliumMainstream Cigarette Smoke (CS) ExposureAirway Epithelial CellsTracheaFully Differentiated Epithelial CellsCalibrated Mainstream CS DeliveryALI Culture SystemRespiratory EpitheliaPhysiological SettingMolecular ResponsesLung Cellular ResponsesEnvironmental Air PollutionHuman HealthChronic Obstructive Pulmonary Disease (COPD)Tobacco-related Diseases
