A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 01:15Dissection of Mouse Tracheas
- 03:40Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
- 08:25Application of Cigarette Smoke to Cultured Epithelial Cells
- 09:44Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
- 10:28Conclusion
Pulmoner farelerin solunum yolu epitel hücreleri izole ve farklılaşmış bir solunum yolu epitel bir model olarak hava-sıvı arayüzü kültüre olabilir. Bu çevresel toksin moleküler yanıtların incelemek üzere bir protokol, tecrit, kültür ve bu hücreler ana sigara dumanına maruz için tarif edilir.
Tags
Mouse Respiratory Epithelial CellsIsolationExperimental Cigarette SmokeAir-liquid Interface (ALI)Differentiated Respiratory EpitheliumMainstream Cigarette Smoke (CS) ExposureAirway Epithelial CellsTracheaFully Differentiated Epithelial CellsCalibrated Mainstream CS DeliveryALI Culture SystemRespiratory EpitheliaPhysiological SettingMolecular ResponsesLung Cellular ResponsesEnvironmental Air PollutionHuman HealthChronic Obstructive Pulmonary Disease (COPD)Tobacco-related Diseases
