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Imaging ottico di neuroni nel ganglio Granchio Stomatogastric con tensione sensibili Coloranti
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
DOI:

09:36 min

March 23, 2011

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Chapters

  • 00:05Title
  • 02:11Preparing the Voltage-Sensitive Dye Solution
  • 03:18Preparing the Stomatogastric Ganglion and the Extracellular Recording
  • 03:58Dye Loading by Intracellular Injection
  • 06:35Imaging of Dyed Neurons
  • 08:03Results: Optical and Electrical Recordings from STG Neurons
  • 08:57Conclusion

Summary

Automatic Translation

Qui vi presentiamo la metodologia per una rapida e ad alta risoluzione fluorescenti tensione-sensibili di imaging tintura di attività dettagliata dei neuroni del ganglio stomatogastric granchio.

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