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1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine,Veterans Administration TVHS
Chapters
- 00:05Title
- 01:30Embryonic Body (EB) Formation Using 96-round Bottom Well Microtiter Plates
- 03:24Identification of Critical Time Windows of Key Signaling Pathway(s) for Cardiogenesis
- 05:04Verification of Time Windows of Signaling for Cardiogenesis in EBs Made from Hanging Dropets
- 07:06Determination of Critical Time Points for ES Cardiogenesis
- 07:45Conclusion
Vi beskriver användningen av en mus ES-cell-baserad analys för att identifiera kritiska tidsfönster för Wnt / β-catenin och BMP signalen ljuder vid kardiogen induktion. Metoden ger en standardiserad plattform som tillförlitligt kvantifierar kardiogen effektivitet, och det är som gäller för studiet av andra cell linjer.
Tags
Modified Mouse Embryonic Stem Cell AssayQuantifying Cardiogenic Induction EfficiencyPluripotent Stem CellsKey Signaling PathwaysTemporal RegulationSpatial RegulationDifferentiation EfficiencyWnt/β-catenin SignalingBMP SignalingContracting Embryonic Bodies96-well Plate FormatCardiogenic EfficiencyModulator TreatmentsTime CourseCell LineagesHigh Throughput AssayAutomated Assay
