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脂质囊泡介导的亲和层析法,使用磁性细胞分选(LIMACS):一种新的方法来分析蛋白质 - 脂质相互作用
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JoVE Journal
Biology
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
Please note that all translations are automatically generated.
Click here for the English version.
脂质囊泡介导的亲和层析法,使用磁性细胞分选(LIMACS):一种新的方法来分析蛋白质 - 脂质相互作用
DOI:
10.3791/2657-v
•
07:33 min
•
April 26, 2011
•
Erhard Bieberich
1
Institute of Molecular Medicine and Genetics
,
Georgia Health Sciences University
Chapters
00:05
Title
01:00
Introduction
01:42
Preparation of Lipid Vesicles and aPKC Binding Assays
04:28
In vitro Lipid-protein Polarity Complex
06:10
LIMACS Purification of PKCζ-EGFP and the Ceramide Binding Domain C20ζ -EGFP
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为了测试我们使用Macs和Annexin V的共轭磁珠和目标脂质和Annexin V的具有约束力的磷脂酰丝氨酸合成脂质囊泡与目标脂质的一种蛋白质的相互作用。绑定到目标脂质蛋白质纯化和分析后,从珠洗脱。
Tags
Lipid Vesicle-mediated Affinity Chromatography
LIMACS
Protein-lipid Interaction
Purification
Lipid ELISA
Plasmon Resonance Spectroscopy
Microdomain Structures
Lipid Binding Properties
Lipid Vesicles
Target Lipid
Phosphatidylserine
Annexin V MACS
Magnetic Beads
Cell Lysate
Co-purification
Recombinant Proteins
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