A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine,University of California at Los Angeles, 2Crump Institute for Molecular Imaging, David Geffen School of Medicine,University of California at Los Angeles, 3California NanoSystems Institute,University of California at Los Angeles, 4Nuclear Medicine, PET Center, Shanghai Medical Collegea,Fudan University, 5Electronics and Information Engineering, College of Electronics and Information Engineering,Wuhan Textile University
Chapters
- 00:05Title
- 01:51Preparation for [18F]SFB Synthesis
- 03:03[18F]SFB Synthesis
- 04:44Preparation of Solid Phase Extraction (SPE)-purified [18F]SFB
- 07:24Representative Results for the Microwave-assisted One-pot Synthesis of [18F]SFB
- 08:10Conclusion
Eine einfache, one-pot Synthese von N-Succinimidyl-4-[<sup> 18</sup> F] fluorbenzoat ([<sup> 18</sup> F] SFB) wurde auf Basis einer nicht-wässrigen, Drei-Stufen-radiochemische Verfahren entwickelt. Durch Mikrowellen-Erhitzung, kann das gesamte Verfahren in weniger als 30 min oder 60 min mit weiteren Reinigung durch präparative HPLC abgeschlossen sein. Der Zerfall korrigierten radiochemischen Ausbeuten (RCYs) waren 35-5% (n> 30).
Tags
Microwave-assistedOne-pot SynthesisN-succinimidyl-4-[18F]fluorobenzoate[18F]SFBBiomoleculesPeptidesProteinsAntibodiesEngineered FragmentsTherapeuticsMolecular Imaging AgentsPositron-emitting RadioisotopesCu-64Ga-68F-18Targeted ImagingPhysiological ProcessesPathological ProcessesSynthesisExploration18F-labeled BiomoleculesDirect 18F-labelingOrganic SolventExtreme PHHigh TemperatureRadiolabeled Prosthetic GroupsMethod Of ChoiceBolton-Hunter Type ReagentPrimary Amino GroupsIn Vivo StabilityRadiolabeling YieldPET Tracers
