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一种简单而有效的隔离混合原代培养成人肝细胞巨噬细胞的方法
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
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一种简单而有效的隔离混合原代培养成人肝细胞巨噬细胞的方法

DOI:
10.3791/2757-v

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08:10 min

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May 24, 2011

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, , , ,
1Transgenic Animal Research Center,National Institute of Agrobiological Sciences, Tsukuba, Japan, 2Safety Research Team,National Institute of Animal Health, Tsukuba, Japan

Chapters

  • 00:05Title
  • 02:14Perfusion of the Liver
  • 03:33Preparation and Culture of Parenchymal Hypatocyte-rich Cell Fraction
  • 04:36Selective Isolation of Macrophages
  • 06:46Results: Highly-purified Rat Liver Macrophages
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

获得原代培养大鼠肝细胞的巨噬细胞的一个新的方法来描述。这种方法利用在文化的巨噬细胞增殖，选择性附件晃动培养瓶和塑料菜的净化。这项技术有效地提供肝巨噬细胞，而无需复杂的设备和技能。

Tags

Isolate MacrophagesMixed Primary CulturesAdult Liver CellsKupffer CellsLiver-specific Resident MacrophagesIsolation MethodsSophisticated EquipmentCentrifugal ElutriatorTechnical SkillsNovel MethodSufficient Number And PurityRat Liver CellsTwo-step Perfusion MethodParenchymal HepatocytesCulture MediumDMEMFCSEpithelial Cell MorphologyFibroblast-like CellsPhase Contrast-bright Round Macrophage-like CellsProliferation Of Macrophage-like CellsShaking Of Culture FlasksSuspension Of MacrophagesTransfer And Short Incubation In Plastic Dishes

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