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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母
DOI:
10.3791/2779-v
•
10:27 min
•
July 18, 2011
•
Dalibor Mijaljica
,
Mark Prescott
,
Rodney J. Devenish
1
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences
,
Monash University
Chapters
00:05
Title
01:33
Preparation of Yeast Strains
03:00
Cell Growth and Induction of Mitophagy by Nitrogen Starvation
04:48
Labeling of the Vacuole with CMAC-Arg
06:26
Mounting Live Yeast Cells for Fluorescence Microscopy
07:52
Visualizing Mitophagy by Fluorescence Microscopy
10:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个强大的方法来监视传递到细胞器的降解和回收的酵母液泡酸性流明描述。该方法依赖一种基因编码的双发射荧光pH值传感器,和可视化的单个细胞,用荧光显微镜对特定目标细胞器的标签。
Tags
Fluorescence Microscopy
Mitophagy
Yeast Saccharomyces Cerevisiae
Autophagy
Cellular Turnover
Organelle Degradation
Mitochondria
Homeostasis
Quality Control Mechanism
Fluorescence Reporter
Rosella
PH-sensitive Green Fluorescent Protein
PH Differences
Vacuole
Nitrogen Starvation
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剖析
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酿酒酵母</em
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微阵列分析FOR
酿酒酵母</em
裂殖酵母的活细胞定量荧光显微镜分析
致命的甲壳类动物长期毒性试验
卤虫franciscana</em
囊性纤维化跨膜传导调节蛋白表达和纯化
酿酒酵母
可视化和分析的mRNA分子,使用荧光<em>在原位</em>杂交<em>酿酒酵母(Saccharomyces cerevisiae)</em
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