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染色质免疫沉淀从以下轴索损伤的背根神经节组织

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury

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染色质免疫沉淀从以下轴索损伤的背根神经节组织

DOI:

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09:41 min

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July 20, 2011

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Elisa Floriddia*^1,2, Tuan Nguyen*¹, Simone Di Giovanni

¹Laboratory for NeuroRegeneration and Repair, Department of Neurology, Hertie Institute for Clinical Brain Research,University of Tuebingen , ²Graduate School for Cellular and Molecular Neuroscience,University of Tuebingen

Chapters

00:05Title
01:04Sciatic & Dorsal Column Nerve Injury
04:35Cross-linking
06:14Immunoprecipitation and Elution
08:23Increased Expression After Nerve Injury
09:11Conclusion

Summary

Automatic Translation

我们目前从背根神经节组织中的染色质免疫沉淀性轴索损伤的方法。这种方法可以用来识别特定的转录因子结合位点，同时在周边及中枢神经系统受伤的轴突再生的表观遗传修饰的组蛋白与DNA的重要。

Tags

Chromatin Immunoprecipitation Dorsal Root Ganglia Axonal Injury Central Nervous System Peripheral Nervous System Neurite Outgrowth Axonal Regeneration Transcriptional Programs Gene Regulation Injury Model System Transcriptional Activity Animal Removal Nerve Fibers Sciatic Nerve Spinal Cord Transcription Factor Binding Histone Modification DNA Modification ChIP Protocol

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