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加上质谱肽免疫亲和富集的蛋白质定量
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
加上质谱肽免疫亲和富集的蛋白质定量
DOI:
10.3791/2812-v
•
06:09 min
•
July 31, 2011
•
Lei Zhao*
1
,
Jeffrey R. Whiteaker*
1
,
Matthew E. Pope
,
Eric Kuhn
,
Angela Jackson
,
N. Leigh Anderson
,
Terry W. Pearson
,
Steven A. Carr
,
Amanda G. Paulovich
1
Clinical Research Division
,
Fred Hutchinson Cancer Research Center – FHCRC
,
2
Department of Biochemistry and Microbiology
,
University of Victoria
,
3
Broad Institute of MIT and Harvard
,
4
Genome BC Proteomics Centre
,
University of Victoria
,
5
Plasma Proteome Institute
Chapters
00:05
Title
01:05
Trypsin Enzymatic Digestion and Cleanup
03:17
Peptide Immunoaffinity Enrichment
04:45
Analysis by Multiple Reaction Monitoring – Mass Spectrometry
05:24
MRM of a SISCAPA-Enriched Sample
05:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
稳定同位素标准和捕捉反肽抗体(SISCAPA)夫妇亲和富集稳定同位素稀释质谱(MRM - MS)的肽作为代理人为各自的蛋白质肽的定量测量提供。在这里,我们描述了一个部分自动化的格式使用磁性粒子的协议。
Tags
Quantification
Proteins
Peptide Immunoaffinity Enrichment
Mass Spectrometry
Traditional Immunoassays
SISCAPA
Stable Isotope Standards
Capture By Anti-peptide Antibodies
SRM/MRM-MS
Small Molecules
Protein Concentration
Proteotypic Peptides
Trypsin
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