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Detection of Neuritic Plaques in Alzheimer’s Disease Mouse Model
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Detection of Neuritic Plaques in Alzheimer’s Disease Mouse Model

Detection of Neuritic Plaques in Alzheimer’s Disease Mouse Model

DOI:
10.3791/2831-v

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06:02 min

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July 26, 2011

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, ,
1Department of Neuroscience,The University of British Columbia

Chapters

  • 00:05Title
  • 01:18Fixation of Mouse Brains and Cryosectioning
  • 02:14Immunohistochemistry Procedure for Neuritic Plaques
  • 03:44Thioflavin S Staining Procedure for Detection of Neuritic Plaques
  • 04:38Valproic Acid Inhibition of Neuritic Plaque Formation

Summary

Automatic Translation

Automatic Translation

One of the pathological characteristics of AD is the formation of Amyloid β protein positive neuritic plaques. In this protocol we describe two methods to detect neuritic plaques in transgenic AD model mice: immunohistochemical detection using the ABC and DAB method and fluorescent detection using thioflavin S staining method.

Tags

Neuritic PlaquesAlzheimer’s DiseaseMouse ModelDetectionAmyloid Beta ProteinAPP Processing PathwayImmunohistochemical MethodABC MethodDAB Method

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