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Journal
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Immunology and Infection
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测量细菌感染小鼠和免疫反应
李斯特菌</em
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Measuring Bacterial Load and Immune Responses in Mice Infected with
Listeria monocytogenes
Please note that all translations are automatically generated.
Click here for the English version.
测量细菌感染小鼠和免疫反应
李斯特菌</em
DOI:
10.3791/3076-v
•
12:50 min
•
August 09, 2011
•
Nancy Wang
,
Richard Strugnell
,
Odilia Wijburg
,
Thomas Brodnicki
1
St Vincent’s Institute, Department of Medicine
,
The University of Melbourne
,
2
Department of Microbiology and Immunology
,
The University of Melbourne
Chapters
00:05
Title
01:08
Preparation of Listeria Inoculum and Intravenous Injection of Mice
04:13
Removal of Liver and Spleen from Listeria-infected Mice for Analysis
05:57
Preparation of Hepatic Cell Suspensions for FACS Analysis
08:40
Measurement of Bacterial Load in Tissues from Listeria-infected Mice
10:26
Representative Data for Bacterial Load and Immune Cell Analysis
12:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
李斯特菌
是一种模式生物,为研究免疫反应和遗传易感性细胞在小鼠体内的细菌。这种方法使一个衡量细菌负荷,产生单细胞悬液,流式细胞仪分析小鼠肝和脾,以确定
由于李斯特
菌感染的免疫细胞的变化。
Tags
Bacterial Load
Immune Responses
Mice
Listeria Monocytogenes
Gram-positive
Intracellular Pathogen
Systemic Infection
Spleen
Liver
CD8α+ Dendritic Cells
Kupffer Cells
Phagocytosed
Bacterial Proteins
Phagosome
Cytosol
Innate Immune Cells
Monocytes
Neutrophils
NK Cells
Dendritic Cells
Bactericidal Mechanisms
CD8+ T Cells
Clearance Of Listeria
Host Immune Responses
Inbred Mouse Strains
Genetic Studies
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