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JoVE Journal
Immunology and Infection

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一个用于标识Allelotyping PCR扩增沙门氏菌肠炎血清型,哈达尔,海德堡和鼠伤寒沙门氏菌
 
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一个用于标识Allelotyping PCR扩增沙门氏菌肠炎血清型,哈达尔,海德堡和鼠伤寒沙门氏菌

Article DOI: 10.3791/3130
July 22nd, 2011

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Summary July 22nd, 2011

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Click here for the English version.

我们描述了一个多重PCR快速检测沙门氏菌血清型肠炎,哈达尔,海德堡和鼠伤寒沙门氏菌。具体的沙门氏菌血清型,可确定的目标是多重PCR技术,以独特的O -抗原生物合成集群和一个给定的血清型的鞭毛的基因和序列。血清型,然后分配到一个隔离基础上的具体化,规模扩增产物(PCR产物)对应的目标等位基因外观的沙门氏菌。

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