/
/
תא מבוססי סידן Assay עבור בינוני עד הקרנת תפוקה גבוהה של פונקציות TRP באמצעות ערוץ FlexStation 3
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:41Cell Preparation
- 02:52Calcium Assay Preparation
- 04:37Plate Reading in FlexStation 3 and Data Analysis
- 05:48Representative Calcium Assay Results
- 07:05Conclusion
Tags
Cell-based Calcium AssayMedium To High Throughput ScreeningTRP Channel FunctionsFlexStation 3Multi-mode Microplate ReaderFluorescence MeasurementMulti-well PlatesAcademic SettingsIntegrated Fluid Transfer ModuleMulti-channel PipetterFluorescence ChangesCa2+-permeable Ion ChannelsG-protein Coupled ReceptorsIntracellular Free Ca2+ LevelsTransient Receptor Potential (TRP) ChannelsCalcium InfluxTRPA1 ChannelMolecular SensorNoxious StimuliHEK293 CellsCa2+-sensitive Fluorescent DyeFluo-4Real-time Fluorescence ChangesTRPA1 AgonistTRPA1 Antagonist
