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Chapters
- 00:05Title
- 01:34Day 1: Viral Infection and Preparation of Buffers
- 02:56Day 2, Phase 1: Cell Lysis and Ultracentrifugation
- 04:01Day 2, Phase 2: Ultracentrifugation
- 07:35“Ghost” DNA Precipitation
- 09:56Representative Results
- 11:51Conclusion
Noi descriviamo il processo di isolamento del DNA ad alta herpesvirus nucleocapside purezza dalle cellule infette. Il DNA finale catturato dalla soluzione è di alta concentrazione e purezza, rendendolo ideale per high-throughput sequencing, l'alta fedeltà reazioni di PCR, e trasfezioni per produrre nuovi ricombinanti virali.
Tags
Viral DNANucleocapsidsHost Cell ContaminantsDownstream ApplicationsViral Genome SequencingHost DNA RemovalViral Recombinant StrainsPurified PlasmidLinear Viral DNAExtractionsDensity-based CentrifugationPurified Viral CapsidsCellular ProteinsOD260/280 RatioYields Of Viral DNALong-term StorageHigh-throughput SequencingHigh Fidelity PCR ReactionsTransfections
