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1Diabetes and Obesity Research Center,Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center,Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology,Indiana University School of Medicine
Chapters
- 00:05Title
- 01:23Surgery
- 05:21Hyperinsulinemic-euglycemic Clamp
- 09:09Evaluation of Insulin Action in Chow-fed vs. High Fat-fed Obese Mice using the Hyperinsulinemic-euglycemic Clamp
- 10:34Conclusion
El clamp hiperinsulinémico euglucémico, o una pinza de insulina, es el estándar de oro para evaluar la acción de la insulina<em> En vivo</em>. Un método para la realización de las abrazaderas de insulina en ratones se ha descrito. Esto incluye un método para la cateterización arterial, que permite los experimentos que se realizaron en ratones conscientes, sin trabas, con un mínimo de estrés.
Tags
Hyperinsulinemic-euglycemic ClampsInsulin ActionType 2 DiabetesInsulin InfusionGlucose InfusionGlucose Infusion Rate (GIR)Whole-body Insulin ActionIsotopic 2[14C]deoxyglucose[3-3H]glucoseEndogenous Glucose Appearance (endoRa)Hepatic Glucose ProductionWhole-body Glucose Disappearance (Rd)Genetic Mouse Models Of Metabolic DiseaseDiabetes ResearchInsulin Clamp Performance
