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分离小鼠原代滋养细胞及滋养细胞侵袭实验

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Biology
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JoVE Journal Biology

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

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分离小鼠原代滋养细胞及滋养细胞侵袭实验

DOI:

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10:28 min

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January 08, 2012

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Kathleen A. Pennington, Jessica M. Schlitt, Laura C. Schulz

¹Obstetrics, Gynecology and Women’s Health,University of Missouri

Chapters

00:05Title
01:30Dissection of the Mouse Placenta
03:29Isolation of Trophoblast Cells
06:53Trophoblast Invasion Assay
08:21Representative Results

Summary

Automatic Translation

在这个协议中，我们描述了剥离的胎盘从怀孕d10.5，将鼠标放在隔离的滋养层细胞，用Percoll梯度。然后，我们演示如何使用分离的细胞在基底膜侵袭实验。

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