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マウスの主栄養膜細胞と絨毛浸潤アッセイの分離
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Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
Please note that all translations are automatically generated.
Click here for the English version.
マウスの主栄養膜細胞と絨毛浸潤アッセイの分離
DOI:
10.3791/3202-v
•
10:28 min
•
January 08, 2012
•
Kathleen A. Pennington
,
Jessica M. Schlitt
,
Laura C. Schulz
1
Obstetrics, Gynecology and Women’s Health
,
University of Missouri
Chapters
00:05
Title
01:30
Dissection of the Mouse Placenta
03:29
Isolation of Trophoblast Cells
06:53
Trophoblast Invasion Assay
08:21
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルでは、パーコール勾配を用いた栄養膜細胞の分離に続いて妊娠d10.5、上のマウスから胎盤の解剖について説明します。次に、マトリゲル浸潤アッセイで単離された細胞の使用例を示します。
Tags
Isolation
Primary Mouse Trophoblast Cells
Trophoblast Invasion Assay
Placenta
Nutrients
Gasses
Growth Factors
Wastes
Placental Development
Fetus
Mother
Embryonic Lethality
Cell Type
Fetal Portion
Tool
Normal Placental Development
Abnormal Placental Development
Cell Lines
Specific Stages Of Pregnancy
Primary Cultures
Transgenic Mice
Particular Genes
Percoll Gradient
Trophoblast Cell Population
Mouse Placentas
Human Trophoblast Cell Isolation
Purity Assessment
Immunocytochemical Staining
Cytokeratin 74
Matrigel Invasion Assay
Trophoblast Invasiveness In Vitro
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