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Chapters
- 00:05Title
- 01:30Dissection of the Mouse Placenta
- 03:29Isolation of Trophoblast Cells
- 06:53Trophoblast Invasion Assay
- 08:21Representative Results
En este protocolo, se describe la disección de placenta de ratón en el embarazo D10.5, seguido por el aislamiento de las células trofoblásticas utilizando un gradiente de Percoll. A continuación, demostrar el uso de las células aisladas en un ensayo de invasión de Matrigel.
Tags
IsolationPrimary Mouse Trophoblast CellsTrophoblast Invasion AssayPlacentaNutrientsGassesGrowth FactorsWastesPlacental DevelopmentFetusMotherEmbryonic LethalityCell TypeFetal PortionToolNormal Placental DevelopmentAbnormal Placental DevelopmentCell LinesSpecific Stages Of PregnancyPrimary CulturesTransgenic MiceParticular GenesPercoll GradientTrophoblast Cell PopulationMouse PlacentasHuman Trophoblast Cell IsolationPurity AssessmentImmunocytochemical StainingCytokeratin 74Matrigel Invasion AssayTrophoblast Invasiveness In Vitro
