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Chapters
- 00:05Title
- 01:10Printing Custom miRNA Microarrays and Sample Preparation
- 02:23Microarray Hybridization
- 04:32Post Hybridization Processing
- 06:03Representative Microarray Results
- 06:39Conclusion
En simpel procedure til at udføre brugerdefinerede microRNA microarray eksperimenter er beskrevet. Trinene omfatter isolere RNA, mærkning RNA og reference DNA, hybridizing prøverne til microarrays, scanning af microarrays, kvantificere og analysere hybridisering signaler.
Tags
Custom MicroRNA MicroarrayMiRNAsRNA MoleculesTarget GenesPost-transcriptionallyBiological ProcessesAltered MiRNA ExpressionHuman DiseasesBiomarkersPrognosisMiRNA Expression ProfilingReal-time PCRDeep SequencingMiRNA Microarray TechniqueHigh-throughputLess ExpensiveMolecular Biology LaboratoryUniversitiesMedical SchoolsHospitalsCustom MiRNA Microarray ExperimentsGlass SlidesRNA IsolationFluorescence Dye
