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Chapters
- 00:05Title
- 01:10Printing Custom miRNA Microarrays and Sample Preparation
- 02:23Microarray Hybridization
- 04:32Post Hybridization Processing
- 06:03Representative Microarray Results
- 06:39Conclusion
Um procedimento simples de realizar experimentos de microarray personalizado microRNA é descrito. As etapas incluem isolar RNA, rotulagem RNA e DNA de referência, hibridação as amostras para microarrays, a digitalização da microarrays, quantificar e analisar os sinais de hibridização.
Tags
Custom MicroRNA MicroarrayMiRNAsRNA MoleculesTarget GenesPost-transcriptionallyBiological ProcessesAltered MiRNA ExpressionHuman DiseasesBiomarkersPrognosisMiRNA Expression ProfilingReal-time PCRDeep SequencingMiRNA Microarray TechniqueHigh-throughputLess ExpensiveMolecular Biology LaboratoryUniversitiesMedical SchoolsHospitalsCustom MiRNA Microarray ExperimentsGlass SlidesRNA IsolationFluorescence Dye
