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Utføre Custom mikroRNA Microarray Experiments
JoVE Journal
Biology
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JoVE Journal Biology
Performing Custom MicroRNA Microarray Experiments
DOI:

07:04 min

October 28, 2011

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Chapters

  • 00:05Title
  • 01:10Printing Custom miRNA Microarrays and Sample Preparation
  • 02:23Microarray Hybridization
  • 04:32Post Hybridization Processing
  • 06:03Representative Microarray Results
  • 06:39Conclusion

Summary

Automatic Translation

En enkel prosedyre å utføre tilpassede mikroRNA microarray eksperimenter er beskrevet. Trinnene omfatter isolere RNA, merking RNA og referanse DNA, hybridizing prøvene til microarrays, skanning microarrays, kvantifisere og analysere hybridisering signaler.

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